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The Method of Analysis of Hypericin (HPLC)
Wave length: 230 nm
Chromatography column:Kromasil C18
Column oven temp:40℃
2.Preparation of Standard solution
Weigh accurately about 0.5 mg Hypericin standard sample, transfer to a 10 ml volumetric flask. Make up the volume to the mark with Methanol.
3.Preparation of Sample solution
Weigh accurately about 0.1g Hypericin sample, transfer to a 10 ml volumetric flask. Add appropriate amount Methanol dissolve by sonication. When the temperature of the solution return to the room temperature, make up the volume to the mark with Methanol.Centrifugate the solution,use the Oil system filters to filter supernatant.
A1:Sample peak area
C:Standard sample concentration
V: Sample dilution
A2:Standard Sample peak area
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